Human Collagen IV

Mouse

Human, Primate (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Unmodified

Purified human Collagen IV

• IHC - Paraffin (1:10 - 1:50)
• IHC - Frozen (1:10 - 1:50)
• Western blot (1:10 - 1:50)

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.

PBS, pH 7.3, 0.09% Sodium Azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

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