Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C744025-1 1 ml (100 µg/ml) $437 
Antibody

Monoclonal Mouse anti‑Human Collagen IV Antibody (IHC, WB) LS‑C744025

Monoclonal Mouse anti‑Human Collagen IV Antibody (IHC, WB) LS‑C744025

Antibody:
Collagen IV Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, WB
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$437
LS-C744025-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Collagen IV Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, WB
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IV antibody LS-C744025 is an unconjugated mouse monoclonal antibody to Collagen IV from human. It is reactive with human and primate. Validated for IHC and WB.
Target
Human Collagen IV
Host
Mouse
Reactivity
Human, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified human Collagen IV
Applications
  • IHC - Paraffin (1:10 - 1:50)
  • IHC - Frozen (1:10 - 1:50)
  • Western blot (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.
Presentation
PBS, pH 7.3, 0.09% Sodium Azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/20/2022