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Catalog Number Size Price
LS-C744058-0.5 0.5 ml (1 mg/ml) $987 
Collagen III Antibody - Collagen Type III on chicken skin paraffin section DAB, hematoxylin

Polyclonal Rabbit anti‑Chicken Collagen III Antibody (IHC, IF, WB) LS‑C744058

Polyclonal Rabbit anti‑Chicken Collagen III Antibody (IHC, IF, WB) LS‑C744058

Antibody:
Collagen III Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA, RIA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$987
LS-C744058-0.5
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Collagen III Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA, RIA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen III antibody LS-C744058 is an unconjugated rabbit polyclonal antibody to chicken Collagen III. Validated for ELISA, IF, IHC, RIA and WB.
Target
Chicken Collagen III
Host
Rabbit
Reactivity
Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified collagen type III from chicken skin
Applications
  • IHC - Paraffin (1:500)
  • Immunofluorescence (1:80)
  • Western blot
  • ELISA (1:200)
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type III 100%, chicken collagen type I <5%.
Presentation
PBS
Reconstitution
0.5 ml Reconstitute in sterile distilled water.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/4/2022