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Catalog Number Size Price
LS-C744048-0.5 0.5 ml (1 mg/ml) $879 
Collagen II Antibody - Collagen Type II on rat cartilage paraffin section DAB, hematoxylin

Polyclonal Rabbit anti‑Rat Collagen II Antibody (IHC, IF) LS‑C744048

Polyclonal Rabbit anti‑Rat Collagen II Antibody (IHC, IF) LS‑C744048

Antibody:
Collagen II Rabbit anti-Rat Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$879
LS-C744048-0.5
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Collagen II Rabbit anti-Rat Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen II antibody LS-C744048 is an unconjugated rabbit polyclonal antibody to rat Collagen II. Validated for ELISA, IF, IHC and RIA.
Target
Rat Collagen II
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified collagen type II from fetal rat cartilage
Applications
  • IHC - Paraffin (1:100 - 1:500)
  • Immunofluorescence (1:40)
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type II 100%, rat collagen I, III and V <0.1%; rat elastin, rat-fibronectin <0.1% (solid phase RIA at 1:100 dilution).
Presentation
PBS
Reconstitution
0.5 ml Reconstitute in sterile distilled water.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/20/2022