After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type II 100%, rat collagen I, III and V <0.1%; rat elastin, rat-fibronectin <0.1% (solid phase RIA at 1:100 dilution).
0.5 ml Reconstitute in sterile distilled water.
For research use only. Intended for use by laboratory professionals.
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