The antibody reacts with native as well as with heat denatured human collagen types I, II, III, in RIA, ELISA. The antibody does not react with other collagen types (IV and V). Human plasma proteins do not interfere with binding to collagen.
For staining of tissue sections it is advised to use acetone or ethanol-fixed cryostat sections, results of staining of paraffin embedded tissues even after treatment with proteolytic enzymes or microwave are less satisfactory. For ELISA dilute 1:1000, for histochemistry dilute 1:20.
This antibody carries the LSBio 100% Guarantee.