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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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COL6A1 / Collagen VI Alpha 1 Antibody (aa831‑858) LS‑C99321
Collagen VI Alpha 1 antibody LS-C99321 is an unconjugated rabbit polyclonal antibody to human Collagen VI Alpha 1 (COL6A1). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C99321-400
400 µl
$305
Description
Collagen VI Alpha 1 antibody LS-C99321 is an unconjugated rabbit polyclonal antibody to human Collagen VI Alpha 1 (COL6A1). Validated for Flow, IF, IHC and WB.
Target
Human COL6A1 / Collagen VI Alpha 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8314 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99321. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa831-858
Specificity
This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-858 amino acids from the C-terminal region of human COL6A1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About COL6A1 / Collagen VI Alpha 1
P12109 NM_001848 NP_001839.2

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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.
Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.
Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.
Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from A431 cell line, using COL6A1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.
Western blot of COL6A1 antibody in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/16/2018
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