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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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COL4A6 / Collagen IV Antibody (aa822‑871, FITC) LS‑C467021
Collagen IV antibody LS-C467021 is an FITC-conjugated rabbit polyclonal antibody to Collagen IV (COL4A6) from human, chimpanzee and gibbon. Validated for WB.
Catalog
Size
Price
LS-C467021-100
100 µl (1 mg/ml)
Unavailable

Popular COL4A6 / Collagen IV Products

IHC of paraffin-embedded human cervix carcinoma tissue, using Collagen IV alpha6 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Western blot
Immunofluorescent analysis of Collagen 4 alpha 6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Monkey, Rat
Applications: ICC, Immunofluorescence, Western blot
Western blot of COL4A6 antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
IHC-P: Collagen IV antibody testing of human lung cancer tissue
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Collagen IV antibody LS-C467021 is an FITC-conjugated rabbit polyclonal antibody to Collagen IV (COL4A6) from human, chimpanzee and gibbon. Validated for WB.
About COL4A6 / Collagen IV
COL4A6 / Collagen IV is one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Q14031 NM_001847 NP_001838.2

COL4A6 Antibody, Collagen, type IV, alpha 6 Antibody, Collagen alpha 6 type IV Antibody, Collagen alpha 6(iv) Antibody, Collagen alpha-6(IV) chain Antibody, CXDELq22.3 Antibody, DELXq22.3 Antibody


Specifications

Target
Human COL4A6 / Collagen IV
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa822-871 of human COL4A6 (Q14031, NP_001838). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey (92%).
Epitope
aa822-871
Specificity
Human COL4A6
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS.
Storage
Short term, 4°C. Long term, add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A6 antibody LS-C135695 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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