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Antibodies > COL2A1 / Collagen II Alpha 1 > LS-C800256
 
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Catalog Number Size Price
LS-C800256-100 100 µl (1 mg/ml) $379 
LS-C800256-200 200 µl (1 mg/ml) $421 
COL2A1 / Collagen II Alpha 1 Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
COL2A1 / Collagen II Alpha 1 Antibody - 1:200 staining human liver cells by IF/ICC. cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 min at room temperature. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
COL2A1 / Collagen II Alpha 1 Antibody - 1:200 staining human liver cells by IF/ICC. cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 min at room temperature. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
COL2A1 / Collagen II Alpha 1 Antibody - Western blot analysis on COLO205 cell lysates using Collagen II antibody. The lane on the left is treated with the antigen-specific peptide.
COL2A1 / Collagen II Alpha 1 Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
COL2A1 / Collagen II Alpha 1 Antibody - 1:200 staining human liver cells by IF/ICC. cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 min at room temperature. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
COL2A1 / Collagen II Alpha 1 Antibody - 1:200 staining human liver cells by IF/ICC. cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 min at room temperature. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
COL2A1 / Collagen II Alpha 1 Antibody - Western blot analysis on COLO205 cell lysates using Collagen II antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human COL2A1 / Collagen II Alpha 1 Antibody (IHC, IF, WB) LS‑C800256

Polyclonal Rabbit anti‑Human COL2A1 / Collagen II Alpha 1 Antibody (IHC, IF, WB) LS‑C800256

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
COL2A1 / Collagen II Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800256-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COL2A1 / Collagen II Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen II Alpha 1 antibody LS-C800256 is an unconjugated rabbit polyclonal antibody to Collagen II Alpha 1 (COL2A1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human COL2A1 / Collagen II Alpha 1
Synonyms
COL2A1 | Alpha-1 type II collagen | Cartilage collagen | COL11A3 | Collagen alpha 1(ii) | Collagen, type II, alpha 1 | Collagen alpha-1(II) chain | Chondrocalcin | ANFH | AOM | SEDC
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Collagen II.
Specificity
Collagen II antibody detects endogenous levels of total Collagen II.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL2A1 / Collagen II Alpha 1
P02458 NM_001844 NP_001835.3

LSBio Ratings

COL2A1 / Collagen II Alpha 1 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C800256 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8. Yeyang Wang, Xiaoyu Zheng, Dixin Luo, Wangyang Xu, Xiaozhong Zhou. BMC musculoskeletal disorders. 2022 September;23:872.
Full Text Article PubMed: 36127685 PMC: PMC9487131
Submit a Publication for LS-C800256

Customer Reviews (0)

Submit a review for LS-C800256

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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
COL2A1 / Collagen II Alpha 1 Antibody - Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
COL2A1 / Collagen II Alpha 1 Antibody (N‑Terminus) LS‑C354627
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
COL2A1 / Collagen II Alpha 1 Antibody - Western blot of COL2A1 antibody
COL2A1 / Collagen II Alpha 1 Antibody (70‑150 aa, N‑terminal) LS‑C382807
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
COL2A1 / Collagen II Alpha 1 Antibody - Collagen II antibody IHC-frozen: Rat Trachea Tissue.
COL2A1 / Collagen II Alpha 1 Antibody (aa1222‑1241) LS‑C343923
Species: Mouse, Rat
Applications: IHC, IHC - Frozen, Western blot
Product
COL2A1 / Collagen II Alpha 1 Antibody (clone 2F12D3) LS‑C765694
Species: Mouse, Rat
Applications: IHC
COL2A1 / Collagen II Alpha 1 Antibody - Western blot of COL2A1 / Collagen II Alpha 1 antibody.
COL2A1 / Collagen II Alpha 1 Antibody (aa1386‑1467, Biotin) LS‑C687751
Species: Human
Applications: Western blot
See all COL2A1 / Collagen II Alpha 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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