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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CNTN1 / gp135 / Contactin 1 Antibody (aa313‑324) IHC‑plus™ LS‑B10585
Note: This antibody replaces LS-C204997
Contactin 1 antibody LS-B10585 is an unconjugated goat polyclonal antibody to Contactin 1 (CNTN1 / gp135) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10585-50
50 µg (0.5 mg/ml)
$425

Popular CNTN1 / gp135 / Contactin 1 Products

CNTN1 / Contactin 1 antibody LS-C116853 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: Western blot
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10586 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CNTN1 / Contactin 1 antibody LS-C116853 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: Western blot

Product Description

CNTN1 / gp135 / Contactin 1 Antibody (aa313-324) for IHC, WB/Western IHC-plus™ LS-B10585

Specifications

Target
Human CNTN1 / gp135 / Contactin 1
Synonyms
CNTN1, Contactin, Contactin 1, Glycoprotein gp135, gp135, Neural cell surface protein F3, Contactin-1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-ENIRGKDKHQAR from an internal region of human CNTN1 / gp135 / Contactin 1 (NP_001834.2; NP_778203.1; NP_001242992.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Pig, Guinea pig (100%); Ferret, Bovine, Panda (92%); Elephant, Rabbit, Horse, Turkey, Zebra finch, Chicken, Platypus (83%).
Epitope
aa313-324
Specificity
Human CNTN1 / gp135 / Contactin 1. This antibody is expected to recognize all reported isoforms (NP_001834.2; NP_778203.1; NP_001242992.1). Reported variants represent identical protein: NP_001242993.1, NP_001242992.1.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Human, Mouse and Rat Brain lysates (calculated MW of 113kD according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CNTN1 / gp135 / Contactin 1
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Q12860 NM_001843 NP_001834.2


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Images

Immunohistochemistry

Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.

Western blot

CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.

Western blot

CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.

Western blot

CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10585 concentration 5 ug/ml.

Western blot

CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 10/18/2018

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