Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Contactin 1 antibody LS-B10585 is an unconjugated goat polyclonal antibody to Contactin 1 (CNTN1 / gp135) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Synthetic peptide C-ENIRGKDKHQAR from an internal region of human CNTN1 / gp135 / Contactin 1 (NP_001834.2; NP_778203.1; NP_001242992.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Pig, Guinea pig (100%); Ferret, Bovine, Panda (92%); Elephant, Rabbit, Horse, Turkey, Zebra finch, Chicken, Platypus (83%).
Human CNTN1 / gp135 / Contactin 1. This antibody is expected to recognize all reported isoforms (NP_001834.2; NP_778203.1; NP_001242992.1). Reported variants represent identical protein: NP_001242993.1, NP_001242992.1.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Human, Mouse and Rat Brain lysates (calculated MW of 113kD according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus.