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Antibodies > CNOT8 > LS-C99242
 
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Catalog Number Size Price
LS-C99242-400 400 µl (0.4 mg/ml) $393 
CNOT8 Antibody - Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CNOT8 Antibody - Fluorescent confocal image of HeLa cell stained with CNOT8 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
CNOT8 Antibody - CNOT8 Antibody western blot of U251 cell line lysates (35 ug/lane). The CNOT8 antibody detected the CNOT8 protein (arrow).
CNOT8 Antibody - Flow cytometric of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CNOT8 Antibody - Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CNOT8 Antibody - Fluorescent confocal image of HeLa cell stained with CNOT8 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
CNOT8 Antibody - CNOT8 Antibody western blot of U251 cell line lysates (35 ug/lane). The CNOT8 antibody detected the CNOT8 protein (arrow).
CNOT8 Antibody - Flow cytometric of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human CNOT8 Antibody (aa227‑255, IHC, IF, WB) LS‑C99242

Polyclonal Rabbit anti‑Human CNOT8 Antibody (aa227‑255, IHC, IF, WB) LS‑C99242

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
CNOT8 Rabbit anti-Human Polyclonal (aa227-255) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C99242-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CNOT8 Rabbit anti-Human Polyclonal (aa227-255) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CNOT8 antibody LS-C99242 is an unconjugated rabbit polyclonal antibody to human CNOT8 (aa227-255). Validated for Flow, IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human CNOT8
Synonyms
CNOT8 | CAF1-like protein | CALIFp | CCR4-associated factor 8 | CAF2 | HCAF1 | POP2 | CAF1 | CALIF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa227-255
Specificity
This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CNOT8
Q9UFF9 NM_004779 NP_004770.4

LSBio Ratings

CNOT8 Antibody (aa227-255) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-C99242 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay. Hyerim Yi, Joha Park, Minju Ha, Jaechul Lim, Hyeshik Chang, V Narry Kim. Molecular cell. 2018 Jun;70:1081-1088e5.
Full Text Article PubMed: 29932901
Submit a Publication for LS-C99242

Customer Reviews (0)

Submit a review for LS-C99242

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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