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Catalog Number Size Price
LS-C288817-10 10 µl $295 
LS-C288817-100 100 µl (1 mg/ml) $514 
CML / BCR Antibody - Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
CML / BCR Antibody - Flow Cytometric Detection of BCR. One million K562 cells were fixed, permeabilized, and stained with 0.25 ug anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR (black).
CML / BCR Antibody - Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
CML / BCR Antibody - Flow Cytometric Detection of BCR. One million K562 cells were fixed, permeabilized, and stained with 0.25 ug anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR (black).
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Polyclonal Rabbit anti‑Human CML / BCR Antibody (aa1221‑1271, IF, WB) LS‑C288817

Polyclonal Rabbit anti‑Human CML / BCR Antibody (aa1221‑1271, IF, WB) LS‑C288817

Antibody:
CML / BCR Rabbit anti-Human Polyclonal (aa1221-1271) Antibody
Application:
ICC, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C288817-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CML / BCR Rabbit anti-Human Polyclonal (aa1221-1271) Antibody
Application:
ICC, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BCR antibody LS-C288817 is an unconjugated rabbit polyclonal antibody to human BCR (CML) (aa1221-1271). Validated for Flow, ICC, IF, IP and WB.
Target
Human CML / BCR
Synonyms
BCR | Acute lymphocytic leukemia | ALL | BCR1 | BCR/FGFR1 chimera protein | Breakpoint cluster region | Chronic myeloid leukemia | D22S11 | CML | D22S662 | Philadelphia chromosome | PHL | FGFR1/BCR chimera protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 613 corresponding to amino acids between 1221 and 1271 of UniprotID: P11274
Epitope
aa1221-1271
Specificity
Region between residue 1221 and 1271 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Applications
  • ICC (1:500 - 1:2000)
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (10 µg/mg lysate)
  • Flow Cytometry
Usage
IHC-IF: 1:500-1:2000 Flo: 0.5 µg per 1 X 10^6 cells in a 150 µl volume
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CML / BCR
P11274 NM_004327 NP_004318.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024