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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CMA1 / Mast Cell Chymase Antibody IHC‑plus™ LS‑B12242
Note: This antibody replaces LS-C25450
Mast Cell Chymase antibody LS-B12242 is an unconjugated mouse monoclonal antibody to human Mast Cell Chymase (CMA1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B12242-250
250 µl (0.08 mg/ml)
$485
Description
Mast Cell Chymase antibody LS-B12242 is an unconjugated mouse monoclonal antibody to human Mast Cell Chymase (CMA1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CMA1 / Mast Cell Chymase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified human skin chymase. Cellular Localization: Cytoplasmic.
Specificity
Recognizes human mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CMA1 / Mast Cell Chymase
P23946 NM_001836 NP_001827.1

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Images

Immunohistochemistry - Paraffin

Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Requested From: United States
Date Requested: 12/11/2018
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