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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CMA1 / Mast Cell Chymase Antibody (aa164‑213) IHC‑plus™ LS‑B13110
Note: This antibody replaces LS-C135687
Mast Cell Chymase antibody LS-B13110 is an unconjugated rabbit polyclonal antibody to Mast Cell Chymase (CMA1) from human, dog and guinea pig. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B13110-50
50 µg
$485
Description
Mast Cell Chymase antibody LS-B13110 is an unconjugated rabbit polyclonal antibody to Mast Cell Chymase (CMA1) from human, dog and guinea pig. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CMA1 / Mast Cell Chymase
Host
Rabbit
Reactivity
Human, Dog, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa164-213 of human CMA1 (P23946, NP_001827). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Dog (100%); Marmoset (92%); Rat, Sheep, Elephant, Panda, Bat, Guinea pig (85%).
Epitope
aa164-213
Specificity
Human CMA1 / Mast Cell Chymase
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CMA1 / Mast Cell Chymase
P23946 NM_001836 NP_001827.1

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Images

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CMA1 / Mast Cell Chymase antibody western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/15/2018
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