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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CLIC4 Antibody LS‑C413641
CLIC4 antibody LS-C413641 is an unconjugated rabbit polyclonal antibody to CLIC4 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C413641-100
100 µl (1 mg/ml)
$275
Description
CLIC4 antibody LS-C413641 is an unconjugated rabbit polyclonal antibody to CLIC4 from human, mouse and rat. Validated for IHC and WB.
Target
Human CLIC4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CLIC4 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence of human CLIC4.
Specificity
Recognizes endogenous levels of CLIC4 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CLIC4
Q9Y696 NM_013943 NP_039234.1

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Images

Immunohistochemistry

Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.
Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.
Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.
Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of CLIC4 staining in human breast cancer;rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.
Western blot analysis of CLIC4 expression in mouse brain (A); rat brain (B) whole cell lysates.

Requested From: United States
Date Requested: 12/18/2018
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