Mouse CLDN5 / Claudin 5

Mouse

Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide derived from the mouse Claudin-5 protein.

Recognizes rat endogenous Claudin-5 protein at ~22-24kD. Species cross-reactivity: WB: mouse, IHC: human.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Western blot (1 - 3 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00501 NCBI: NM_003277 NP_003268.2