Human CLDN12 / Claudin 12

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the N-Terminus of mouse and human Claudin 12

N-Terminus

Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

• IHC
• IHC - Paraffin (5 µg/ml)
• Immunofluorescence (5 µg/ml)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry: LS-B2181 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2181 was determined to be 5 ug/ml. Immunofluorescence: 5 ug/ml, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P56749 NCBI: NM_012129 NP_036261.1