Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:50000)
CLCN4 / CLC-4 antibody was raised against a 13 AA Peptide sequence near the C-terminus of rat CLC-4 (1). The peptide was coupled to KLH.
The 13 AA rat CLC41-P control peptide is 100% conserved in mouse, rat and human CLC-4. No significant sequence homology is detected with other CLCs or other proteins. Actual cross-reactivity of antibodies in various species has not been studied. There is a very high degree of protein homology among CLC-3, CLC-4 and CLC-5. However, CLC41-P peptide has very little sequence homology with CLC-3 and CLC-5. Anti-rat CLC41 antibodies were checked against the corresponding CLC-3 and CLC-5. These peptides are also available to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3.