Citrulline coupled to KLH via Glutaraldehyde
The antibody reacts specifically with intrapeptidic citrulline independently of AA sequence
The positive control was HL60 cells treated with DMSO or with DMSO and A23187. The histones of these cells were then extracted.The sample were loaded on a 14% acrylamide SDS PAGE gel.The transfer was made on a PVDF membrane The blocking buffer was PBS 1X with 5% BSA The antibody was used at 1/1000 in a TBT Tween 20 buffer with 1% BSA.The antibody was incubated overnight at +4°C.
100 µl Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.