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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHRM4 / M4 Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A220 Best Seller
M4 antibody LS-A220 is an unconjugated rabbit polyclonal antibody to M4 (CHRM4) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A220-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular CHRM4 / M4 Products

Antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: ICC, Western blot, ELISA
Anti-CHRM4 / M4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: ICC, Western blot, ELISA

Product Description

M4 antibody LS-A220 is an unconjugated rabbit polyclonal antibody to M4 (CHRM4) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CHRM4 / M4
Synonyms
CHRM4, M4 muscarinic receptor, M4R, Muscarinic m4 receptor, HM4, Muscarinic receptor m4
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 - 20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHRM4 / M4 antibody was raised against synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM4. Percent identity with other species by BLAST analysis: Human, Gorilla, Mouse, Rat, Bovine, Hamster, Horse, Pig, Guinea pig (100%); Dog, Elephant, Panda (95%); Bat (90%).
Blocking Peptide
LS-E28521 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A220. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human CHRM4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CHRM4 / M4
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. P08173 NM_000741 NP_000732.2


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Images

Immunohistochemistry

Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 antibody LS-A220 IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM4 / M4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/16/2018

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