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CHRM3 / M3 Antibody (C‑Terminus) LS‑C37395

Catalog Number Size Price
LS-C37395-100 100 µl $435 
Antibody

CHRM3 / M3 Antibody (C‑Terminus) LS‑C37395

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$435 
LS-C37395-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
M3 antibody LS-C37395 is an unconjugated rabbit polyclonal antibody to human M3 (CHRM3 ). Validated for ELISA, IHC and WB.
Target
Human CHRM3 / M3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide analog of the carboxyl terminal of the muscarinic M3 receptor covalently attached onto a carrier protein.
Epitope
C-Terminus
Specificity
Recognizes rat M3 Muscarinic Receptor. Does not recognize M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Specific for the C-terminal of the M3 receptor.
Usage
Western Blot: 1:500Immunohistochemistry: 1:2500 using PLP-fixed rat brain sections (ABC technique)Optimal dilutions to be determined by the researcher.Western Blot Protocol:1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels, such at 12% gels) and electrophoretic transfer to PVDFmembrane, block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.2. Wash 2X with TBS/Tween-20.3. Apply LS-C37395 at dilution of at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 asbuffer for the primary antibody. Incubate for 2-4 hours.4. Wash 3X with TBS/Tween-20.5. Apply affinity purified IgG (HRP) goat x rabbit antiserum (dilution may vary depending upon supplier) in 2% normal goat serum inTBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash 4X for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction.
Presentation
Lyophilized
Reconstitution
100ul PBS, 10mg/ml BSA
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHRM3 / M3
P20309 NM_000740 NP_000731.1

Publications (0)

Reviews (0)

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Requested From: United States
Date Requested: 9/15/2019