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Catalog Number Size Price
LS-C744748-100 100 µg (1 mg/ml) $425 
Choline Oxidase Antibody - Western Blot of Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated. Lane 1: Choline Oxidase (Alcaligenes species). Load: 50 ng per lane. Primary antibody: Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Choline Oxidase
Choline Oxidase Antibody - Goat anti Choline Oxidase antibody was used to detect Choline Oxidase. Samples of ~1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline Oxidase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Choline Oxidase and imaged on the BioRad VersaDoc imaging system.
Choline Oxidase Antibody - Western Blot of Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated. Lane 1: Choline Oxidase (Alcaligenes species). Load: 50 ng per lane. Primary antibody: Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Choline Oxidase
Choline Oxidase Antibody - Goat anti Choline Oxidase antibody was used to detect Choline Oxidase. Samples of ~1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline Oxidase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Choline Oxidase and imaged on the BioRad VersaDoc imaging system.
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Polyclonal Goat anti‑Alcaligenes Choline Oxidase Antibody (Lyophilized, Biotin, WB) LS‑C744748

Polyclonal Goat anti‑Alcaligenes Choline Oxidase Antibody (Lyophilized, Biotin, WB) LS‑C744748

Antibody:
Choline Oxidase Goat anti-Alcaligenes Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Alcaligenes
Format:
Biotin, Lyophilized
Price
Catalog Number
$425
LS-C744748-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Choline Oxidase Goat anti-Alcaligenes Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Alcaligenes
Format:
Biotin, Lyophilized

Specifications

Description
Choline Oxidase antibody LS-C744748 is a biotin-conjugated goat polyclonal antibody to alcaligenes Choline Oxidase. Validated for ELISA, IP and WB.
Target
Alcaligenes Choline Oxidase
Host
Goat
Reactivity
Alcaligenes (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Choline Oxidase [Alcaligenes species].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:2500)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 9/18/2020