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Catalog Number Size Price
LS-C775462-100 100 µg (1 mg/ml) $395 
Choline Acetyltransferase Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Choline Acetyltransferase Antibody - Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
Choline Acetyltransferase Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Choline Acetyltransferase Antibody - Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
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Choline Acetyltransferase Antibody (Atto 700) LS‑C775462

Choline Acetyltransferase Antibody (Atto 700) LS‑C775462

Antibody:
Rabbit Polyclonal to Human Choline Acetyltransferase
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Atto 700, Unmodified
Other formats:
Price
Catalog Number
$395 
LS-C775462-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Choline Acetyltransferase
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Atto 700, Unmodified
Other formats:

Specifications

Description
Choline Acetyltransferase antibody LS-C775462 is an Atto 700-conjugated rabbit polyclonal antibody to Choline Acetyltransferase from human and mouse. Validated for IF and WB.
Target
Human Choline Acetyltransferase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Atto 700. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Immunogen
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
Specificity
Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Choline Acetyltransferase

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Requested From: United States
Date Requested: 10/19/2019