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Catalog Number Size Price
LS-C746740-20 20 µl $275 
LS-C746740-50 50 µl $311 
LS-C746740-100 100 µl $389 
LS-C746740-200 200 µl $503 
CHEK2 / CHK2 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).
CHEK2 / CHK2 Antibody - Immunofluorescence analysis of A549 cells using CHEK2 antibody.
CHEK2 / CHK2 Antibody - Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.
CHEK2 / CHK2 Antibody - Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
CHEK2 / CHK2 Antibody - Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.
CHEK2 / CHK2 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).
CHEK2 / CHK2 Antibody - Immunofluorescence analysis of A549 cells using CHEK2 antibody.
CHEK2 / CHK2 Antibody - Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.
CHEK2 / CHK2 Antibody - Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
CHEK2 / CHK2 Antibody - Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human CHEK2 / CHK2 Antibody (IHC, IF, WB) LS‑C746740

Polyclonal Rabbit anti‑Human CHEK2 / CHK2 Antibody (IHC, IF, WB) LS‑C746740

Antibody:
CHEK2 / CHK2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746740-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CHEK2 / CHK2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CHK2 antibody LS-C746740 is an unconjugated rabbit polyclonal antibody to CHK2 (CHEK2) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB.
Target
Human CHEK2 / CHK2
Synonyms
CHEK2 | BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CHEK2 (NP_009125.1). MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEY
Applications
  • IHC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 15-38kDa/50-65kDa, while the observed MW by Western blot was 60kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK2 / CHK2
O96017 NM_007194 NP_009125.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024