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Catalog Number Size Price
LS-C14446-200 200 µg $650 
Antibody

Monoclonal Mouse anti‑Human CHEK2 / CHK2 Antibody (WB) LS‑C14446

Monoclonal Mouse anti‑Human CHEK2 / CHK2 Antibody (WB) LS‑C14446

Antibody:
Mouse Monoclonal to Human CHEK2 / CHK2
Application:
ICC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-C14446-200
200 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human CHEK2 / CHK2
Application:
ICC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CHK2 antibody LS-C14446 is an unconjugated mouse monoclonal antibody to CHK2 (CHEK2) from human. It is reactive with human, mouse and rat. Validated for ICC, IP and WB.
Target
Human CHEK2 / CHK2
Synonyms
CHEK2 | BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation
Immunogen
Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.
Specificity
Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 ug/ml detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 ul of 2-mercaptoethanol/ 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK2 / CHK2
O96017 NM_007194 NP_009125.1

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Requested From: United States
Date Requested: 7/12/2020