Human CHEK2 / CHK2
CHEK2 | BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.1 - 1 µg/ml)
Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.
Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 ug/ml detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 ul of 2-mercaptoethanol/ 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.