CHK1 antibody LS-C6064 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) from human, mouse, rat and other species. Validated for WB.
Human CHEK1 / CHK1
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
CHEK1 / CHK1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser345 of human Chk1.
Detects endogenous levels of Chk1 only when activated by phosphorylation at serine 345. Does not recognize Chk1 phosphorylated at other sites. Species cross-reactivity: Monkey, mouse and rat.
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.