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Antibodies > CHEK1 / CHK1 > LS-C800867
 
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Catalog Number Size Price
LS-C800867-100 100 µl (1 mg/ml) $379 
LS-C800867-200 200 µl (1 mg/ml) $421 
CHEK1 / CHK1 Antibody - 1:100 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining human lymphoid tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CHEK1 / CHK1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CHEK1 / CHK1 Antibody - Western blot analysis of Chk1 phosphorylation expression in 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CHEK1 / CHK1 Antibody - Western blot analysis of Chk1 phosphorylation expression in 3T3 whole cells lysates. The lane on the right is treated with the antigen-specific peptide.
CHEK1 / CHK1 Antibody - 1:100 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - 1:100 staining human lymphoid tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK1 / CHK1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CHEK1 / CHK1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CHEK1 / CHK1 Antibody - Western blot analysis of Chk1 phosphorylation expression in 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CHEK1 / CHK1 Antibody - Western blot analysis of Chk1 phosphorylation expression in 3T3 whole cells lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (phospho‑Ser301, IHC, IF, WB) LS‑C800867

Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (phospho‑Ser301, IHC, IF, WB) LS‑C800867

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Antibody:
CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer301) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800867-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer301) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CHK1 antibody LS-C800867 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) (pSer301) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CHEK1 / CHK1
Synonyms
CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Chk1 around the phosphorylation site of Serine 301.
Epitope
pSer301
Specificity
Phospho-Chk1 (Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 301.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK1 / CHK1
O14757 NM_001274 NP_001265.2

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Applications: IHC, IHC - Paraffin, Western blot
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CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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