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CHEK1 / CHK1 Antibody (HRP) LS-C498595

Antibody

CHEK1 / CHK1 Antibody (HRP) LS-C498595

Antibody:
Rabbit Polyclonal to Human CHEK1 / CHK1
Application:
ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C498595-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CHEK1 / CHK1
Application:
ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Other formats:

Specifications

Description
CHK1 antibody LS-C498595 is an HRP-conjugated rabbit polyclonal antibody to human CHK1 (CHEK1). Validated for ELISA.

Target
Human CHEK1 / CHK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
CHEK1 / CHK1 antibody was raised against recombinant Human Serine/threonine-protein kinase Chk1 protein (258-370AA)
Specificity
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK1 / CHK1
O14757 NM_001274 NP_001265.2

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Requested From: United States
Date Requested: 6/25/2019
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