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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHEK1 / CHK1 Antibody (aa250-300) LS-C287210

Detection of human CHK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Chk1 antibody used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.
Detection of human CHK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml).
Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Chk1 antibody used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.
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CHEK1 / CHK1 Antibody (aa250-300) LS-C287210

Antibody:
Rabbit Polyclonal to Human CHEK1 / CHK1
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C287210-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CHEK1 / CHK1
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CHK1 antibody LS-C287210 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1). Validated for IHC, IP and WB.

Target
Human CHEK1 / CHK1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHEK1 / CHK1 antibody was raised against portion of GeneID: 1111 corresponding to amino acids between 250 and 300 of UniprotID: O14757
Epitope
aa250-300
Specificity
Region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Usage
IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK1 / CHK1
O14757 NM_001274 NP_001265.2

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Requested From: United States
Date Requested: 6/25/2019
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