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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHAF1A / CAF1 Antibody (clone SS 1 1‑13) IHC‑plus™ LS‑B242 Best Seller
Note: This antibody replaces LS-C2625
CAF1 antibody LS-B242 is an unconjugated mouse monoclonal antibody to human CAF1 (CHAF1A). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B242-50
50 µl
$425

Popular CHAF1A / CAF1 Products

Western blot Image
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

CAF1 antibody LS-B242 is an unconjugated mouse monoclonal antibody to human CAF1 (CHAF1A). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About CHAF1A / CAF1
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. Q13111 NM_005483 NP_005474.2

CHAF1A Antibody, CAF-I p150 Antibody, CAF1P150 Antibody, CAF-1 subunit A Antibody, CAF-1 Antibody, Hp150 Antibody, p150 Antibody, CAF Antibody, CAF-I 150 kDa subunit Antibody, CAF1 Antibody, CAF1B Antibody


Specifications

Target
Human CHAF1A / CAF1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [SS 1 1-13]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHAF1A / CAF1 antibody was raised against recombinant human CAF-1p150
Specificity
Specific for CAF-1p150.
Usage
Immunohistochemistry: LS-B242 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B242 was determined to be 1:50.
Presentation
0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.

Immunohistochemistry

Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.

Immunohistochemistry

Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.

Immunohistochemistry

Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.

Requested From: United States
Date Requested: 9/20/2018

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