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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C15235-100 100 µg $560 
Antibody

Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (N‑Terminus, WB) LS‑C15235

Polyclonal Rabbit anti‑Human CFLAR / FLIP Antibody (N‑Terminus, WB) LS‑C15235

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$560 
LS-C15235-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human CFLAR / FLIP
Application:
ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FLIP antibody LS-C15235 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC and WB.
Target
Human CFLAR / FLIP
Synonyms
CFLAR | C-FLIPR | C-FLIPS | C-FLIP | CASP8AP1 | Cflip | CLARP | CASH | Caspase homolog | Caspase-eight-related protein | Flice inhibitory protein | I-FLICE | Inhibitor of FLICE | FLAME | FLAME1 | MRIT | Usurpin beta | C-FLIPL | Casper | FLAME-1 | FLIP | Usurpin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Immunogen
A 18 residue synthetic peptide based on the N-Terminus of human c-FLIP (aa 2-19) was synthesized and the peptide coupled to KLH. This sequence is identical in all c-FLIP splice variants. The amino acid sequence is approximately 90% identical to that of mouse.
Epitope
N-Terminus
Specificity
This immunoaffinity purified antibody detects 28-58kD proteins, corresponding to the apparent molecular mass of all c-FLIP variants on SDS-PAGE immunoblots, in human, mouse and rat cell lysates and tissue extracts.
Usage
Western Blot: 0.5-1 ug/ml. Immunocytochemistry: 5 ug/ml. Positive control: HeLa Cell Lysate.
Presentation
PBS, pH 6.7-7.0, 0.02% sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFLAR / FLIP
O15519 NM_003879 NP_003870.4

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Requested From: United States
Date Requested: 7/12/2020