FLIP antibody LS-C15235 is an unconjugated rabbit polyclonal antibody to FLIP (CFLAR) from human, mouse and rat. Validated for ICC and WB.
Human CFLAR / FLIP
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- ICC (5 µg/ml)
- Western blot (0.5 - 1 µg/ml)
CFLAR / FLIP antibody was raised against a 18 residue synthetic peptide based on the amino terminus of human c-FLIP (residues 2-19) was synthesized and the peptide coupled to KLH. This sequence is identical in all c-FLIP splice variants. The amino acid sequence is approximately 90% identical to that of mouse.
This immunoaffinity purified antibody detects 28-58kD proteins, corresponding to the apparent molecular mass of all c-FLIP variants on SDS-PAGE immunoblots, in human, mouse and rat cell lysates and tissue extracts.
Western Blot: 0.5-1 ug/ml. Immunocytochemistry: 5 ug/ml. Positive control: HeLa Cell Lysate.
PBS, pH 6.7-7.0, 0.02% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.