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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CFL1 / Cofilin Antibody (phospho‑Tyr140/Ser156) LS‑C93997
Cofilin antibody LS-C93997 is an unconjugated sheep polyclonal antibody to Cofilin (CFL1) from human, mouse and rat. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C93997-500
500 µg (1 mg/ml)
$325

Popular CFL1 / Cofilin Products

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat
Applications: Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat
Applications: Immunofluorescence, Western blot, ELISA
Immunofluorescent analysis of Cofilin staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Pig, Sheep, Zebrafish
Applications: ICC, Immunofluorescence, Western blot
Anti-Cofilin antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7855 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Cofilin antibody LS-C93997 is an unconjugated sheep polyclonal antibody to Cofilin (CFL1) from human, mouse and rat. Validated for IHC and WB.

Specifications

Target
Human CFL1 / Cofilin
Synonyms
CFL1, 18 kDa phosphoprotein, Cofilin, CFL, Cofilin 1 (non-muscle), Cofilin, non-muscle isoform, p18
Host
Sheep
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CFL1 / Cofilin antibody was raised against synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein. The antigen is homologous in rat, mouse and monkey.
Epitope
pTyr140/Ser156
Specificity
Specific for Cofilin-1.
Usage
IHC: Antigen retrieval is essential for use on paraffin sections.
Presentation
Lyophilized
Reconstitution
500 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CFL1 / Cofilin
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. P23528 NM_005507 NP_005498.1


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Requested From: United States
Date Requested: 10/15/2018

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