Complement Factor H antibody LS-B3648 is an unconjugated mouse monoclonal antibody to human Complement Factor H (CFH). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human CFH / Complement Factor H
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot
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Human complement factor H.
Recognizes the human serum complement protein factor H (155kD), binding to a different epitope from MRC OX23. The Recognizes a 43-49kD truncated form of factor H present at low level (1-5 ug/ml) in plasma and urine. In an immunoprecipitation system. Recognizes factor H in other primates, but not in cow, sheep, pig, chicken or rabbit sera. Inhibits the binding of factor H to surface bound C3b, Recognizes Factor H in Western blotting procedures.
Immunohistochemistry: LS-B3648 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3648 was determined to be 10 ug/ml.
0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About CFH / Complement Factor H