Complement Factor H antibody LS-B2585 is an unconjugated goat polyclonal antibody to human Complement Factor H (CFH). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Human CFH / Complement Factor H
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Synthetic peptide C-HLVPDRKKDQYK from an internal region of human CFH / Factor H (NP_000177.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2585. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human CFH / Factor H. This antibody is expected to recognize isoform a (NP_000177.2) only.
Immunohistochemistry: LS-B2585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2585 was determined to be 2.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About CFH / Complement Factor H