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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CFB / Complement Factor B Antibody (aa395‑422) LS‑C100538
Complement Factor B antibody LS-C100538 is an unconjugated rabbit polyclonal antibody to human Complement Factor B (CFB). Validated for IHC and WB.
Catalog
Size
Price
LS-C100538-400
400 µl
$305

Popular CFB / Complement Factor B Products

Anti-CFB / Factor B antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7934 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunodiffusion
Western blot of recombinant CFB / Complement Factor B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Rat
Applications: IHC, Western blot
Western blot of recombinant CFB / Complement Factor B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Complement Factor B antibody LS-C100538 is an unconjugated rabbit polyclonal antibody to human Complement Factor B (CFB). Validated for IHC and WB.

Specifications

Target
Human CFB / Complement Factor B
Synonyms
CFB, AHUS4, B-factor, properdin, C3/C5 convertase, CFAB, C3 proaccelerator, C3 proactivator, Complement factor B, Complement protein Factor B, Glycine-rich beta-glycoprotein, GBG, Factor B, FB, Glycine-rich beta glycoprotein, PBF2, BF, BFD, FBI12, H2-Bf, Properdin factor B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E8889 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100538. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa395-422
Specificity
This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human BF.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CFB / Complement Factor B
CFB / Complement Factor B is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. P00751 NM_001710 NP_001701.2


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Images

Immunohistochemistry

BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.
Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.

Immunohistochemistry

BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.
Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.

Immunohistochemistry

BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.
Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.

Immunohistochemistry

BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BF Antibody (RB18685) IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.
Western blot of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35 ug/lane). BF (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 10/21/2018

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