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Antibodies > CENPQ > LS-C154023
 
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Catalog Number Size Price
LS-C154023-100 100 µg (1.15 mg/ml) $567 
CENPQ Antibody - Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
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Polyclonal Rabbit anti‑Human CENPQ Antibody (IF, WB) LS‑C154023

Polyclonal Rabbit anti‑Human CENPQ Antibody (IF, WB) LS‑C154023

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Antibody:
CENPQ Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154023-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CENPQ Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CENPQ antibody LS-C154023 is an unconjugated rabbit polyclonal antibody to human CENPQ. Validated for ELISA, IF and WB.
Target
Human CENPQ
Synonyms
CENPQ | C6orf139 | Centromere protein Q | CENP-Q
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Applications
  • Immunofluorescence
  • Western blot (1:100 - 1:500)
  • ELISA (1:5000)
Usage
This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kD in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CENPQ
Q7L2Z9 NM_018132 NP_060602.2

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Featured Products

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CENPQ Antibody (clone 2B5‑2D12) LS‑C306654
Species: Human
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CENPQ Antibody LS‑C128072
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
CENPQ Antibody - Immunofluorescence microscopy using the protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide.
CENPQ Antibody LS‑C744974
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
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CENPQ Antibody (PE) LS‑C649282
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Applications: Immunofluorescence, Western blot, ELISA
CENPQ Protein
Human CENPQ Protein (Recombinant His) - LS-G2705
Source: E. coli
Tag: His
CENPQ Protein - 12.5% SDS-PAGE of human C6orf139 stained with Coomassie Blue
Human CENPQ Protein (Recombinant GST) (Full Length) - LS-G34210
Source: Wheat
Tag: GST
See all CENPQ Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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