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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154023-100 100 µg (1.15 mg/ml) $425 
CENPQ Antibody - Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPQ Antibody - Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
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CENPQ Antibody LS‑C154023

CENPQ Antibody LS‑C154023

Antibody:
Rabbit Polyclonal to Human CENPQ
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154023-100
100 µg
1.15 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CENPQ
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CENPQ antibody LS-C154023 is an unconjugated rabbit polyclonal antibody to human CENPQ. Validated for ELISA, ICC, IF and WB.
Target
Human CENPQ
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:100 - 1:500)
  • ELISA
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Specificity
This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.
Usage
This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kD in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CENPQ
Q7L2Z9 NM_018132 NP_060602.2

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Requested From: United States
Date Requested: 11/17/2019