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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CENPI / CENP‑I Antibody (aa50‑100) LS‑C290056
CENP-I antibody LS-C290056 is an unconjugated rabbit polyclonal antibody to human CENP-I (CENPI). Validated for IP and WB.
Catalog
Size
Price
LS-C290056-100
100 µl (1 mg/ml)
$395

Popular CENPI / CENP-I Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot
Western blot of Human Thyroid Tumor. CENPI antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Dog, Horse
Applications: Western blot
Western blot of CENPI / CENP-I antibody.
Species: Mouse
Applications: IHC, Western blot
Western blot of recombinant CENPI / CENP-I.
Species: Human
Applications: IHC, Western blot
Western blot of CENPI / CENP-I antibody.
Species: Mouse
Applications: IHC, Western blot

Product Description

CENP-I antibody LS-C290056 is an unconjugated rabbit polyclonal antibody to human CENP-I (CENPI). Validated for IP and WB.
About CENPI / CENP-I
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone. Q92674 NM_006733 NP_006724.2

CENPI Antibody, CENP-I Antibody, FSH primary response 1 Antibody, Mis6 Antibody, ICEN19 Antibody, LRPR1 Antibody, Centromere protein I Antibody, FSH primary response protein 1 Antibody, FSHPRH1 Antibody


Specifications

Target
Human CENPI / CENP-I
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Epitope
aa50-100
Specificity
Region between residue 50 and 100 of human Centromere Protein I using the numbering given in entry NP_006724.2 (GeneID 2491).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.

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Images

Western blot

Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Western blot

Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Western blot

Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Western blot

Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of Human CENP-I by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-I antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. CENP-I was also immunoprecipitated by rabbit anti-CENP-I antibody BL12216, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Requested From: United States
Date Requested: 9/24/2018

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