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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CENPE Antibody (clone 1H12) LS‑C179700
CENPE antibody LS-C179700 is an unconjugated mouse monoclonal antibody to human CENPE. Validated for ICC.
Catalog
Size
Price
LS-C179700-100
100 µg (1 mg/ml)
Unavailable

Popular CENPE Products

Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Ovarian cancer cells were arrested with Nocodazole before fixation; primary Ab: anti-CenpE mAb (clone 1H12); Secondary Ab: anti-mouse Alexa 488; chromosome visualization: DAPI
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot Image
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, Western blot
Immunohistochemistry of Human thyroid cancer using CENPE Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

CENPE antibody LS-C179700 is an unconjugated mouse monoclonal antibody to human CENPE. Validated for ICC.
About CENPE
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. Q02224 NM_001813 NP_001804.2

CENPE Antibody, CENP-E Antibody, Centromere protein E Antibody, Centromere protein E (312kD) Antibody, Kinesin-related protein CENPE Antibody, PPP1R61 Antibody, KIF10 Antibody, Centromere protein E, 312kDa Antibody, Kinesin family member 10 Antibody


Specifications

Target
Human CENPE
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1H12]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC (10 µg/ml)
Immunogen
CENPE antibody was raised against recombinant full length Human CENP-E.
Specificity
Human CENPE
Presentation
PBS, pH 7.2, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunocytochemistry

Immunocytochemistry

Immunocytochemistry

Immunocytochemistry



Requested From: 
Date Requested: 6/21/2018

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