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Catalog Number Size Price
LS-C59522-100 100 µg (1 mg/ml) $498 
CENPE Antibody - Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.

Monoclonal Mouse anti‑Human CENPE Antibody (clone 1H12, IF, WB) LS‑C59522

Monoclonal Mouse anti‑Human CENPE Antibody (clone 1H12, IF, WB) LS‑C59522

Antibody:
CENPE Mouse anti-Human Monoclonal (1H12) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C59522-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CENPE Mouse anti-Human Monoclonal (1H12) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CENPE antibody LS-C59522 is an unconjugated mouse monoclonal antibody to human CENPE. Validated for ELISA, IF, IP and WB.
Target
Human CENPE
Synonyms
CENPE | CENP-E | Centromere protein E | Centromere protein E (312kD) | Kinesin-related protein CENPE | PPP1R61 | KIF10 | Centromere protein E, 312kDa | Kinesin family member 10
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1H12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
Applications
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:200)
  • ELISA (1:5000 - 1:20000)
Usage
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~320 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CENPE
Q02224 NM_001813 NP_001804.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022