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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CENPE Antibody (aa2573‑2601, PE) LS‑C254619
CENPE antibody LS-C254619 is a PE-conjugated rabbit polyclonal antibody to human CENPE. Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C254619-200
200 µl
$680

Popular CENPE Products

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, Western blot
Ovarian cancer cells were arrested with Nocodazole before fixation; primary Ab: anti-CenpE mAb (clone 1H12); Secondary Ab: anti-mouse Alexa 488; chromosome visualization: DAPI
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot Image
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of Human thyroid cancer using CENPE Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CENPE Antibody (aa2573-2601, PE) for WB/Western, ELISA LS-C254619

Specifications

Target
Human CENPE
Synonyms
CENPE, CENP-E, Centromere protein E, Centromere protein E (312kD), Kinesin-related protein CENPE, PPP1R61, KIF10, Centromere protein E, 312kDa, Kinesin family member 10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with AP, APC, FITC, Biotin, HRP.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Epitope
aa2573-2601
Specificity
This CENPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2573-2601 amino acids from the C-terminal region of human CENPE.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C for up to 6 months. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only.
About CENPE
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. Q02224 NM_001813 NP_001804.2


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Requested From: United States
Date Requested: 10/20/2018

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