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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CENPE Antibody (aa2222‑2361) LS‑C293287 Best Seller
CENPE antibody LS-C293287 is an unconjugated rabbit polyclonal antibody to rat CENPE. Validated for IHC and WB.
Catalog
Size
Price
LS-C293287-20
20 µl (0.5 mg/ml)
$205
LS-C293287-100
100 µl (0.5 mg/ml)
$255
LS-C293287-200
200 µl (0.5 mg/ml)
$295
LS-C293287-1
1 ml (0.5 mg/ml)
$515
LS-C293287-10
10 ml (0.5 mg/ml)
$2,040

Popular CENPE Products

Ovarian cancer cells were arrested with Nocodazole before fixation; primary Ab: anti-CenpE mAb (clone 1H12); Secondary Ab: anti-mouse Alexa 488; chromosome visualization: DAPI
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot Image
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of Human thyroid cancer using CENPE Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CENPE Antibody (aa2222-2361) for IHC, WB/Western LS-C293287

Specifications

Target
Rat CENPE
Synonyms
CENPE, CENP-E, Centromere protein E, Centromere protein E (312kD), Kinesin-related protein CENPE, PPP1R61, KIF10, Centromere protein E, 312kDa, Kinesin family member 10
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, Biotin, FITC, Cy3, HRP, PE, APC, Cy7, APC.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:100 - 1:400)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CENPE antibody was raised against recombinant CENPE (Ala2222-Arg2361) expressed in E. coli.
Epitope
aa2222-2361
Specificity
The antibody is a rabbit polyclonal antibody raised against CENPE. It has been selected for its ability to recognize CENPE in immunohistochemical staining and western blotting.
Usage
ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About CENPE
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. Q02224 NM_001813 NP_001804.2


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Images

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/23/2018

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