LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CENPE Antibody (aa2222‑2361, Biotin) LS‑C684281
CENPE antibody LS-C684281 is a biotin-conjugated rabbit polyclonal antibody to rat CENPE. Validated for IHC and WB.
Catalog
Size
Price
LS-C684281-20
20 µl
$205
LS-C684281-100
100 µl
$265
LS-C684281-200
200 µl
$315
LS-C684281-1
1 ml
$560
LS-C684281-10
10 ml
$2,250

Popular CENPE Products

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, Western blot
Ovarian cancer cells were arrested with Nocodazole before fixation; primary Ab: anti-CenpE mAb (clone 1H12); Secondary Ab: anti-mouse Alexa 488; chromosome visualization: DAPI
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot Image
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of Human thyroid cancer using CENPE Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CENPE antibody LS-C684281 is a biotin-conjugated rabbit polyclonal antibody to rat CENPE. Validated for IHC and WB.
About CENPE
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. Q02224 NM_001813 NP_001804.2

CENPE Antibody, CENP-E Antibody, Centromere protein E Antibody, Centromere protein E (312kD) Antibody, Kinesin-related protein CENPE Antibody, PPP1R61 Antibody, KIF10 Antibody, Centromere protein E, 312kDa Antibody, Kinesin family member 10 Antibody


Specifications

Target
Rat CENPE
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with Biotin, FITC, FITC, Cy3, HRP, PE, APC, Cy7, APC.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CENPE antibody was raised against recombinant CENPE (Ala2222-Arg2361) expressed in E. coli.
Epitope
aa2222-2361
Specificity
The antibody is a rabbit polyclonal antibody raised against CENPE. It has been selected for its ability to recognize CENPE in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CENPE.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CENPE. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 8/18/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy