LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CENPA / CENP‑A Antibody (clone 5A7‑2E11, PE, Atto 594) LS‑C227573
CENP-A antibody LS-C227573 is a PE, Atto 594-conjugated mouse monoclonal antibody to human CENP-A (CENPA). Validated for WB.
Catalog
Size
Price
LS-C227573-100
100 µg (1 mg/ml)
$385

Popular CENPA / CENP-A Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Applications: Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Centromeric Protein A Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of CENPA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

CENP-A antibody LS-C227573 is a PE, Atto 594-conjugated mouse monoclonal antibody to human CENP-A (CENPA). Validated for WB.
About CENPA / CENP-A
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). P49450 NM_001809 NP_001800.1

CENPA Antibody, Centromere autoantigen A Antibody, Centromere-specific histone Antibody, CenH3 Antibody, Centromere protein A Antibody, Centromere protein A, 17kDa Antibody, CENP-A Antibody, Centromere protein A (17kD) Antibody


Specifications

Target
Human CENPA / CENP-A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5A7-2E11]
Conjugations
Phycoerythrin, Atto 594. Also available Unconjugated or conjugated with Atto 488, Atto 390, Atto 565, Atto 633, Atto 594, Atto 655, Atto 680, Atto 700, AP, APC, FITC, Biotin, PerCP, SA, RPE.
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CENPA / CENP-A antibody was raised against synthetic peptide corresponding to a portion of human CENP-A.
Specificity
Recognizes human CENP-A. Detects bands of ~18kD.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images

Western blot

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of CENPA / CENP-A antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 8/17/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy