CENP-A antibody LS-C6047 is an unconjugated rabbit polyclonal antibody to CENP-A (CENPA) from human and monkey. Validated for WB.
Human CENPA / CENP-A
(tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Also available conjugated with AP
- Western blot
- (applications tested for the base form of this product only)
CENPA / CENP-A antibody was raised against synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Rabbit (93%); Nectri (86%).
Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 ug/ml anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 5 mg/ml BSA, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.