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Catalog Number Size Price
LS-C357640-100 100 µg (1 mg/ml) $386 
CEACAM8 / CD66b Antibody - Flow cytometry of CEACAM8 / CD66b antibody

Monoclonal Mouse anti‑Human CEACAM8 / CD66b Antibody (clone ANC1D5) LS‑C357640

Monoclonal Mouse anti‑Human CEACAM8 / CD66b Antibody (clone ANC1D5) LS‑C357640

Antibody:
CEACAM8 / CD66b Mouse anti-Human Monoclonal (ANC1D5) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$386
LS-C357640-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CEACAM8 / CD66b Mouse anti-Human Monoclonal (ANC1D5) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD66b antibody LS-C357640 is an unconjugated mouse monoclonal antibody to human CD66b (CEACAM8). Validated for ELISA and Flow.
Target
Human CEACAM8 / CD66b
Synonyms
CEACAM8 | CD66b antigen | Carcinoembryonic antigen CGM6 | CD67 | CD67 antigen | CGM6 | NCA-95 | CD66b
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
ANC1D5
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant soluble human CD66b.
Specificity
Antibody from clone ANC1D5 binds to CD66b on the cell surface of human PMN in Flow cytometry and detects recombinant CD66b in sandwich EIA utilizing either clone ANC4A4 or ANC3C5 as a capture reagent.
Applications
  • Flow Cytometry
  • ELISA
Usage
Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b antibody(ANC1D5) at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD66b. ELISA: Use for detection.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CEACAM8 / CD66b
P31997 NM_001816 NP_001807.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/8/2021