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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN3 / KAP Antibody (clone 2A5) LS‑C173319
KAP antibody LS-C173319 is an unconjugated mouse monoclonal antibody to human KAP (CDKN3). Validated for Flow and WB.
Catalog
Size
Price
LS-C173319-100
100 µl (0.61 mg/ml)
$335

Popular CDKN3 / KAP Products

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human liver tissue using anti-CDKN3 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry image of paraffin-embedded human breast cancer at a dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Anti-CDKN3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry

Product Description

KAP antibody LS-C173319 is an unconjugated mouse monoclonal antibody to human KAP (CDKN3). Validated for Flow and WB.
About CDKN3 / KAP
CDKN3 encodes a dual specificity protein phosphatase which has been identified as a cyclin-dependent kinase inhibitor and has been shown to interact with, and dephosphorylate human CDK2 kinase. CDKN3 has also been shown to associate with human CDK3 and CDC2. The protein has been reported to remove phosphate from tyrosine residues in model substrates in vitro; however, a mutant protein that bears a lesion in the putative active site cysteine has been shown to lose catalytic activity. Q16667 NM_005192 NP_005183.2

CDKN3 Antibody, CIP2 Antibody, Kinase-associated phosphatase Antibody, KAP Antibody, KAP1 Antibody, CDI1 Antibody, CDK-associated phosphatase Antibody, Kinase associated phosphatase Antibody


Specifications

Target
Human CDKN3 / KAP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2A5]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
CDKN3 / KAP antibody was raised against full length human recombinant protein of human CDKN3(NP_005183) produced in HEK293T cell.
Specificity
Human CDKN3 / KAP
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 9/25/2018

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