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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN2D / p19 INK4d Antibody (clone 2E10) LS‑C196888
P19 INK4d antibody LS-C196888 is an unconjugated mouse monoclonal antibody to human p19 INK4d (CDKN2D). Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C196888-100
100 µg
$355

Popular CDKN2D / p19 INK4d Products

Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Anti-CDKN2D / p19 INK4d antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry analysis of paraffin-embedded human brain, using p19 INK4d Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, Peptide Enzyme-Linked Immunosorbent Assay
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CDKN2D / p19 INK4d Antibody (clone 2E10) for WB/Western, IP, ELISA LS-C196888

Specifications

Target
Human CDKN2D / p19 INK4d
Synonyms
CDKN2D, CDK inhibitor p19INK4d, INK4D, p19, p19 INK4d, p19-INK4D
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [2E10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
CDKN2D (AAH01822, aa1-166 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CDKN2D / p19 INK4d
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About CDKN2D / p19 INK4d
CDKN2D / p19 INK4d is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. P55273 NM_079421 NP_001791.1


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Western blot

Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.
Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.
Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.
Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10.Lane 1: CDKN2D transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.
Immunoprecipitation of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 10/20/2018

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