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p19ARF (Mouse Cdkn2a) Antibody (aa39‑65) LS‑C158187

Catalog Number Size Price
LS-C158187-400 400 µl $325 
Mouse Cdkn2a Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The Cdkn2a antibody detected the Cdkn2a protein (arrow).

p19ARF (Mouse Cdkn2a) Antibody (aa39‑65) LS‑C158187

Antibody:
Rabbit Polyclonal to Mouse p19ARF (Mouse Cdkn2a)
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C158187-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse p19ARF (Mouse Cdkn2a)
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
P19ARF (Mouse Cdkn2a) antibody LS-C158187 is an unconjugated rabbit polyclonal antibody to mouse p19ARF (Mouse Cdkn2a). Validated for WB.
Target
p19ARF (Mouse Cdkn2a)
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E6473 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C158187. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa39-65
Specificity
This Mouse p19ARF / Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the Central region of mouse p19ARF / Cdkn2a (UNIPROT Q64364). Note that the p19ARF sequence is distinct from other mouse and human isoforms of Cdkn2a.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with p19ARF, clone 12-A1-1 antibody, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).
Species: Mouse
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Goat Anti-P19ARF (mouse) Antibody (0.3µg/ml) staining of Mouse (A) and Rat (B) Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
Species: Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Rat
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 9/19/2019