Target
Human CDKN1A / WAF1 / p21
Synonyms
CDKN1A | CDKN1 | CIP1 | CDK-interacting protein 1 | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k + IgG2b,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.
Specificity
Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.
Applications
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 ug immunoprecipitates Cip1 from 500 ug of HeLa nuclear extract. Western Blot Analysis: 0.5-2 ug/ml detects WAF1, p21 in 20 ug of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 ug per lane for minigels.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.5% sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDKN1A / WAF1 / p21