P21 antibody LS-C18529 is an unconjugated mouse monoclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated for IP and WB.
Human CDKN1A / WAF1 / p21
(tested or 100% immunogen sequence identity)
IgG1,k + IgG2b,k
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.
Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.
Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 ug immunoprecipitates Cip1 from 500 ug of HeLa nuclear extract. Western Blot Analysis: 0.5-2 ug/ml detects WAF1, p21 in 20 ug of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 ug per lane for minigels.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.5% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About CDKN1A / WAF1 / p21