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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1A / WAF1 / p21 Antibody LS‑C18529
P21 antibody LS-C18529 is an unconjugated mouse monoclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated for IP and WB.
200 µg

Popular CDKN1A / WAF1 / p21 Products

Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Formalin-fixed, paraffin-embedded human colon cancer stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunoprecipitation
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
p21 antibody WA-1 immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Western blot analysis of extracts of various cell lines.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot

Product Description

P21 antibody LS-C18529 is an unconjugated mouse monoclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated for IP and WB.
About CDKN1A / WAF1 / p21
CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P38936 NM_078467 NP_000380.1

CDKN1A Antibody, CDKN1 Antibody, CIP1 Antibody, CDK-interacting protein 1 Antibody, DNA synthesis inhibitor Antibody, p21CIP1 Antibody, MDA6 Antibody, PIC1 Antibody, SDI1 Antibody, WAF1 Antibody, CAP20 Antibody, CDK-interaction protein 1 Antibody, MDA-6 Antibody, p21 Antibody


Human CDKN1A / WAF1 / p21
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k + IgG2b,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein G purified
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag.
Recognizes WAF1, p21,~21kD. Species cross-reactivity: mouse.
Suitable for use in Immunoprecipitation, Western Blot. Immunoprecipitation: 4 ug immunoprecipitates Cip1 from 500 ug of HeLa nuclear extract. Western Blot Analysis: 0.5-2 ug/ml detects WAF1, p21 in 20 ug of HeLa nuclear extract. HeLa nuclear extract was resolved by SDS PAGE, transferred to nitrocellulose, and probed with anti-WAF1, p21 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: HeLa nuclear extract. Use 20 ug per lane for minigels.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.5% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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