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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1A / WAF1 / p21 Antibody (clone CIP1/823, Ready‑to‑Use) LS‑C389948
P21 antibody LS-C389948 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C389948-7000
7000 µl
$395
Description
P21 antibody LS-C389948 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF, IHC and WB.
Target
Human CDKN1A / WAF1 / p21
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG2a,k Monoclonal [CIP1/823]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Ready-to-Use. Also available Unmodified or Azide-free.
Applications
  • IHC
  • IHC - Paraffin (1:1)
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Full-length Human Protein.
Specificity
Human CDKN1A / WAF1 / p21
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Optimal dilution of the p21 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Presentation
PBS, 0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About CDKN1A / WAF1 / p21
P38936 NM_078467 NP_000380.1

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Applications: IHC, IHC - Paraffin, Immunoprecipitation
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Immunohistochemistry Image
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p21 antibody WA-1 immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Formalin-fixed, paraffin-embedded bladder carcinoma stained with p21 antibody (HJ21).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma (SCC) stained with p21 antibody (CIP1/823). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/18/2018
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