Human CDKN1A / WAF1 / p21

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG2a,k Monoclonal

CIP1/823

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.

Recombinant Full-length Human Protein.

Human CDKN1A / WAF1 / p21

• IHC
• IHC - Paraffin (2 - 4 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Optimal dilution of the p21 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P38936 NCBI: NM_078467 NP_000380.1