LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CDKN1A / WAF1 / p21 Antibody (aa58‑77) LS‑C17111
P21 antibody LS-C17111 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C17111-50
50 µg
$530

Popular CDKN1A / WAF1 / p21 Products

Formalin-fixed, paraffin-embedded human colon cancer stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunoprecipitation
Western blot blot of extracts of various cell lines, using CDKN1A antibody.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
p21 antibody WA-1 immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded bladder carcinoma stained with p21 antibody (HJ21).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry

Product Description

P21 antibody LS-C17111 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF, IHC, IP and WB.
About CDKN1A / WAF1 / p21
CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P38936 NM_078467 NP_000380.1

CDKN1A Antibody, CDKN1 Antibody, CIP1 Antibody, CDK-interacting protein 1 Antibody, DNA synthesis inhibitor Antibody, p21CIP1 Antibody, MDA6 Antibody, PIC1 Antibody, SDI1 Antibody, WAF1 Antibody, CAP20 Antibody, CDK-interaction protein 1 Antibody, MDA-6 Antibody, p21 Antibody


Specifications

Target
Human CDKN1A / WAF1 / p21
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence (1 - 5 µg/ml)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length recombinant human p21Cip1/WAF1.
Epitope
aa58-77
Specificity
Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
Usage
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Flow Cytometry: 2 ug/ml. Western Blot (chemiluminescence): 0.1-1 ug/ml. Immunohistochemistry (frozen/paraffin): 5 ug/ml. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 ug/IP reaction. Immunoprecipitates p21 but not associated proteins.
Presentation
Supplied s a liquid in PBS, pH 7.4, 0.1% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 9/20/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy