Human CDKN1A / WAF1 / p21
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence (1 - 5 µg/ml)
- Western blot
- Flow Cytometry (2 µg/ml)
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Full length recombinant human p21Cip1/WAF1.
Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Flow Cytometry: 2 ug/ml. Western Blot (chemiluminescence): 0.1-1 ug/ml. Immunohistochemistry (frozen/paraffin): 5 ug/ml. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 ug/IP reaction. Immunoprecipitates p21 but not associated proteins.
Supplied s a liquid in PBS, pH 7.4, 0.1% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.